HPLC fingerprint and multi-index content determination of children antifebrile lotion / 中草药

ABSTRACT

Objective: To establish HPLC fingerprint of antipyretic lotion for children and determine the content of 10 components (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythiae A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, rosmarinic acid and forsythin). Methods: The analysis of antipyretic lotion for children was performed on the Kromasil 100-5 C18 (250 mm × 4.6 mm, 5 μm) chromatographic column, with mobile phase comprising of 0.1% phosphate acid-acetonitrile flowing at 1 mL/min in a gradient elution manner, and the column temperature was 35 ℃, and detection wavelength was set at 330 nm. With forsythia A as reference peak, HPLC fingerprint of 11 batches of preparations was established; Based on the conditions of fingerprint chromatogram, the content of 10 components was determined at the detection wavelength of 330 and 280 nm and the multi-index content of 11 batches of the preparation was determined. Results: HPLC fingerprint was established, a total of 37 peaks were selected as the common peaks, of which 22 peaks were identified, and the similarities of 11 batches of preparations were between 0.987 and 0.999. The linear relationships of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythiae A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, rosmarinic acid, and forsythioside were good in the range of 14.18-141.78, 20.53-205.63, 14.38-143.78, 5.62-56.19, 22.22-222.22, 8.40-83.98, 5.70-57.02, 7.46-76.36, 16.95-169.48, 8.59-85.94 g/mL, respectively. The average recoveries were 109.51%, 98.73%, 99.41%, 90.63%, 92.73%, 95.39%, 91.87%, 106.50%, 95.23%, and 108.71%. The content of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, forsythiae A, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, rosmarinic acid, and forsythin in the 11 batches were in the range of 306.38-457.85, 607.67-854.71, 306.81-469.02, 95.65-170.64, 484.41-819.44, 234.28-322.01, 145.42-226.85, 219.11-292.21, 347.94-507.74, 201.35-261.94 mg/mL, respectively. Conclusion: The method is accurate, simple, stable and reliable, which can be used for the quality control of antipyretic lotion for children.