Effect of berberine combined with 6-shogaol on notch signaling pathway in colon epithelial cells of ulcerative colitis mice / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3147-3154, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-851024
ABSTRACT
Objective:
To investigate the effects of berberine and 6-shogaol on Notch signaling pathway in colonic epithelial cells of mice with ulcerative colitis.Methods:
A total of 50 Kunming mice were randomly selected as normal group, and the remaining 40 mice were treated with po 2% DSS to induce ulcerative colitis. The rats were randomly divided into model group, berberine group, 6-shogaol group, and berberine + 6-shogaol group. The corresponding drugs were given by ig administration. Colon tissues were taken after 20 d of administration. The protein expression of Hes-1, Math-1, MUC2, and olfm4 in colon epithelium was detected by immunofluorescence method, and the gene and protein expression of Notch-1, Hes-1, Math-1 in colon epithelium were detected by qRT-PCR and Western blotting.Results:
Compared with the model group, the protein and mRNA expression of Notch-1, Hes-1, and olfm4 in colon epithelium of mice in 6-shogaol group, berberine group, and berberine + 6-shogaol group were significantly decreased, while the expression of Math-1 and MUC2 were significantly increased. Among three groups, berberine + 6-shogaol group showed the best effect.Conclusion:
Both 6-shogaol and berberine can effectively repair damaged colonic mucosa and treat ulcerative colitis. The combined use of berberine and 6-shogaol is more effective than the single application. Its mechanism is related to inhibiting the over-activation of Notch pathway and regulating the proliferation and differentiation of colonic epithelium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2019
Type:
Article
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