Metabonomics study of MCAO rats based on microdialysis technique combined with UPLC-Q/TOF-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3155-3161, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-851025
ABSTRACT
Objective:
To study the brain striatum metabolomics characteristics of middle cerebral artery occlusion (MCAO) rats.Methods:
The middle cerebral artery was occluded by monofilament to establish the MCAO rat model. The microdialysis probe was implanted into the striatum, and the brain microdialysis samples were collected under awake and free activity condition and were measured by UPLC-Q/TOF-MS in positive and negative ion mode respectively. Multivariate statistics were used to establish the metabobomics model.Results:
Brain-targeted metabolomics studies based on microdialysis samples were successfully performed. Metabolomics models could clearly distinguish normal and model rats, and seven differential metabolites were identified.Conclusion:
The brain metabolism characteristics of MCAO rats had changed significantly. The metabolomics research based on microdialysis technology enriches the research methods of metabolomics.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2019
Type:
Article
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