Determination of multi-marker components and HPLC fingerprint for quality evaluation of multiple stems Paris polyphylla var. yunnanenesis / 中草药

ABSTRACT

Objective: HPLC was used to determine the content of the polyphyllins VII, H, VI, II, III, I, and V in the rhizomes of multiple stems Paris polyphylla var. yunnanenesis from different places of origin, establishing HPLC fingerprint and comparative analysis HPLC fingerprint between multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis. Methods: HPLC was performed on the column of Thermore C18 with mobile phase of acetonitrile-water gradient at the flow rate of 1 mL/min; The detection wave-length was 203 nm, column temperature was 30 ℃, and volume was 10 μL; Content of multiple stems P. polyphylla var. yunnanenesis. was measured by external standard and quantitative analysis of multi-components (QAMS), and one-way ANOVA was used to explore its content difference. The HPLC fingerprint of rhizome from multiple stems P. polyphylla var. yunnanenesis was established and compared with that of rhizome from P. polyphylla var. yunnanensis. Results: The determination results of 10 batches of rhizome of multiple stems P. polyphylla var. yunnanenesIs showed a good linear relationship in the linear range (r > 0.999 7), with an average recovery of 98.34%-99.34% and RSD ≦ 1.00%. There was a significant difference (P < 0.05) or highly significant difference (P < 0.01) about content of polyphyllins VII, H, VI, II, III, I, and V among different places of origin in the rhizomes of multiple stems P. polyphylla var. yunnanenesis, the total content of polyphyllins (I+ II + VI + VII)% ranging from 1.239%-6.236%, which was significantly higher than 0.60% of the quality control standard of Paridis Rhizoma prescribed by Chinese Pharmacopoeia. No significant difference was observed between the results of external standard and quantitative analysis of multi-components methods. The HPLC fingerprint similarity evaluation results showed that there were 12 common fingerprint peaks between multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis, and with the high similarity of HPLC fingerprints. Conclusion: The method was simple, accurate and reproducible, and can be used for the determination of polyphyllins VII, H, VI, II, III, I, and V in multiple stems of P. polyphylla var. yunnanenesis, total content of polyphyllins (I + II + VI + VII)% in multiple stems of P. polyphylla var. yunnanenesis was relatively higher and HPLC fingerprints of rhizome of multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis from different places of origin also have high similarity.