Simultaneous determination of eight components before and after processing of Gentiana crassicaulis by UPLC-Q-Orbitrap HRMS / 中草药

ABSTRACT

Objective: To develop and validate a ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) Methods: for simultaneously qualitative and quantitative determination of eight chemical components (gentiopicroside, loganic acid, swertiamarin, sweroside, luteolin, isovitexin, apigenin, and kaempferol) in raw and processed products of Gentiana crassicaulis. Methods Waters Acquity UPLC BEH C18 (100 mm × 2.1 mm, 1.7 μm) column was used for gradient elution with methanol-0.1% formic acid in mobile phase. The volume flow was 0.3 mL/min and the column temperature was 35 ℃. The mass spectrometer was detected using negative ion detection mode. Results: After frying and wine frying, the content of iridoid in G. crassicaulis was significantly increased. Statistically, the differences between gentiopicroside and loganic acid after frying were significant. The content of flavonoids changed little after frying and wine frying. The total content changes of the two components were as follows the content of iridoids frying > wine frying > raw products; flavonoids content raw products > wine frying > frying. Conclusion: Based on UPLC-Q-Orbitrap HRMS, the quantitative analysis method of eight components of raw and processed products of G. crassicaulis was established, which provided a basis for optimizing the processing technology of G. crassicaulis and improving the clinical curative effect of G. crassicaulis.