1H-NMR based metabolomic study of sedative and hypnotic effect of Ziziphi Spinosae Semen in rats / 中草药

ABSTRACT

Objective: 1H-NMR based metabolomic approach has been employed to study the intervention effects of Ziziphi Spinosae Semen (ZSS) on p-chlorophenylalanine (PCPA)-induced insomnia using a rat model. Methods 1H-NMR analysis was used to identify the various compounds of ZSS, and the quantitative data were further processed by principal component analysis (PCA), partial least squares discrimination analysis (PLS-DA), and orthogonality PLS-DA to find potential biomarkers. In addition, MetPA analysis was used for pathway analysis. Results:Multivariate analysis revealed that 11 perturbed endogenous metabolites in the serum could be reversed by ZSS at dosage of 15 g/kg and 30 g/kg, and MetPA analysis revealed that the anti-hypnotic effect of ZSS was probably related with regulation of glutamate and glutamite metabolism, taurine and taurine metabolism, alanine, aspartic acid and glutamate metabolism, glyoxylic acid, and dicarboxylic acid metabolism, glycine, serine and threonine metabolism, pyruvate metabolism, tryptophan metabolism, and tricarboxylic acid cycle. ZSS at dosage of 15 g/kg and 30 g/kg both can significant callback 11 potential biomarkers. Conclusion: These metabolic changes indicated that ZSS facilitated recovery from insomnia by regulating the amino acid metabolism and energy metabolism, which provides the basis for elucidating therapeutic mechanisms underlying the efficiency of ZSS for insomnia treatment.