Establishment and application of fingerprint and quantitative method of volatile components in Shuangyu Granules / 中草药

ABSTRACT

Objective To establish the GC fingerprint and components quantitative determination of Shuangyu Granules (SG) for quality control. Methods Agilent DB-1 capillary column (30 m × 0.32 mm, 0.25 μm) was used with upper column volume of 1 μL and split ratio of 25∶1; Injection port was 230 ℃ and detector temperatures was 250 ℃; The carrier gas was nitrogen and its flow rate was 0.6 mL/min. The column temperature was raised by program. Results The fingerprints with good separation and repeatability included 12 mutual common peaks, and the similarity of each batch was more than 0.95. Seven chemical components were identified by GC/MS and standard compounds, which were eucalyptol (peak 2), camphor (peak 3), menthone (peak 4), borneol (peak 5), menthol (peak 6), pulegone (peak 9), and undecanone (peak 10); Among which, peaks of 1, and 10—12 were from Houttuyniae Herba, peaks of 2 and 5 from Artemisiae Argyi Folium and Menthae Haplocalycis Herba, peak of 3 from Artemisiae Argyi Folium, peak of 4, 6, 8 and 9 from Menthae Haplocalycis Herba, peak of 7 from Menthae Haplocalycis Herba, Artemisiae Argyi Folium and Houttuyniae Herba. Moreover, eucalyptol and menthol were quantified and the average recovery rates of eucalyptol and menthol were 103.9% and 98.2% (RSD were 2.1% and 2.3%), respectively. The content of eucalyptol and menthol in 10 batches samples were 0.17—0.23 mg/g and 0.45—0.67 mg/g, respectively. Conclusion The method of fingerprint and quantitative analysis of two components in SG was rapid, simple, and accurate, which could be used for the quality control of SG.