An exploration on mechanisms of “treating different diseases with same method” of Xiaoyao Powder in treating depression and diabetes based on network pharmacology / 中草药

ABSTRACT

Objective: In this paper, herb-component-common target network and protein interaction network were constructed to study the mechanism of Xiaoyao Powder in the treatment of depression and diabetes for exploring the common mechanism of Xiaoyao Powder in treating these two diseases. Methods: TCM database@Taiwan, TCMID, Batman-TCM database and literature mining were used to obtain the components and targets of Xiaoyao Powder, combined with CTD, TTD, PharmGKB, OMIM, Gencards, and Drugbank databases to obtain the targets of Xiaoyao Powder in the treatment of depression and diabetes. The herb-component-common target network was constructed by Cytoscape software. The tool of Metascape was used to perform GO biological processes analysis, Reactome pathway analysis, KEGG pathway analysis, protein interaction networks construction and module analysis on the common targets of Xiaoyao Powder in the treatment of depression and diabetes. BioGPS database and Genecards database were used to analyze the tissue distribution and subcellular distribution of the hub targets, and the tissue-target network and subcellular-target network were constructed by Cytoscape. Protein class was performed on hub pathway targets by DisGeNET database. Results: The results showed that the immune and inflammatory responses, G protein-coupled receptors, insulin and insulin receptors, and monoamine neurotransmitter were involved in the treatment of these two diseases by regulating cAMP signaling pathway, Calcium signaling pathway and PI3K-Akt signaling pathway by Xiaoyao Powder. Conclusion: It provides a direction for further research on Xiaoyao Powder’s common mechanism in treating depression and diabetes. It suggests that the efficacy of Xiaoyao Powder in the treatment of depression and diabetes are mainly involved with the regulation of BDNF-related signaling pathways, the process of G protein-coupled receptors, monoamine neurotransmitter and the insulin and insulin receptors, which will benefit to the further investigation on the declaration of the characteristics of the pharmacological mechanism of Xiaoyao Powder.