Preparation and anticancer effect of natural active compound usenamine nano-liposomes / 中草药

ABSTRACT

Objective To study the antitumor effect of natural active compound usenamine (C18H17NO6, 6-acety1-2- (1-amino-ethylidene)-7,9-dihydroxy-8,9b-dimethy1-9bH-dibenzofuran-1,3-dione) nano-liposomes in vitro and in vivo. Methods Nano-liposomes were prepared by thin film dispersion-ultrasonic method. The drug loading of nano-liposomes was determined by HPLC. The cancer cells were cultured in vitro, and the absorbance values were measured by MTT method to evaluate the anticancer effect in vitro. A nude mouse xenograft model was established to observe the growth inhibitory effect of drug-loaded nano-liposomes on tumor growth in nude mice. Results The drug loading of drug-loaded nano-liposomes was 1.26 mg/mL. The IC50 of the drug-loaded nano-liposomes in the XWLC-05, HCT-116, and HepG2 cells were 2.48 μg/mL, 0.86 μg/mL, and 1.86 μg/mL, respectively, and the inhibition rates of XWLC-05, HCT-116, and HepG2 cells administered at a dose of 4 μg/mL were 85.59%, 99.95%, and 96.91%, respectively (P < 0.01). The minimum relative tumor proliferation rate of nude mice was 50.98%, and usenamine nano-liposomes had antitumor effect in vivo. Conclusion The natural active compound usenamine can be prepared as a nano-liposome.In vitro cell experiments showed that usenamine nano-liposomes had a dose-effect relationship with cancer cells. In vivo experiments in nude mice found that drug-loaded nano-liposomes had inhibitory effect on tumors.