Effect of glaucocalyxin A on proliferation and cell cycle of triple-negative breast cancer MDA-MB-231 cells / 中草药

ABSTRACT

Objective: To investigate the effect of glaucocalyxin A on proliferation and cell cycle of triple-negative breast cancer MDA-MB-231 cells and its mechanism. Methods The proliferation inhibition rates of MDA-MB-231 cells were measured by MTT assay. The cell cycle was analyzed by flow cytometry, and the expression of the protein cyclin B1, cyclin D1, CDK2, CDK4, p53, p21, p27, LSD1, H3K4me2, and H3K9me2 was detected by Western blotting. Results Growth of MDA-MB-231 cells was significantly inhibited by glaucocalyxin A in a dose-dependent and time-dependent manner. Flow cytometric analysis indicated that the percentage of MDA-MB-231 cells at G2/M phase was increased significantly. As the results of Western blotting, the protein expression levels of p53, p21, p27, H3K4me2, and H3K9me2 in MDA-MB-231 cells were increased, while that of cyclin B1, cyclin D1, CDK2, CDK4, and LSD1 were decreased after treated with glaucocalyxin A. Conclusion Glaucocalyxin A could inhibit the proliferation of MDA-MB-231 cells and induce cell cycle arrest at the G2/M phase, and the mechanism may be related to the activation of p53 protein expression and the regulation of histone methylation.