Adsorption properties of ginkgolic acid on molecularly imprinted polymer prepared by using salicylic acid as dummy template / 中草药

ABSTRACT

objective To develop a new method for the adsorption and separation of ginkgolic acid (GA). Methods Using salicylic acid (SA) as a dummy template and 4-vinylpyridine as the functional monomer, molecularly imprinted polymer (MIP) with high adsorbability to GA was synthesized by molecular self-assembly technique. The imprinting mechanism of the polymers was studied by 1H-NMR and IR spectra, and the structure of the polymers was characterized by SEM. The structure of the polymer was analyzed by FT-IR and the adsorption and binding properties of the polymer to total GA were analyzed by HPLC and UV detection. Results MIP had better three-dimensional space structure and adsorption properties. Template molecules were binded to functional monomers with noncovalent bonds. The adsorption rate of GA by polymer MIP in the extract of Ginkgo biloba was 95.9%. The Scatchard analysis reveals that there were two different recognition sites in MIP in the extract of G. biloba, and the apparent maximal combination amount (Qmax1) was 30 mg/g in high affinity recognition sites and with (Qmax2) = 80 mg/g in low affinity recognition sites. And the adsorption kinetics can be best described as the pseudo-second-order kinetics model. Conclusion The preparation of MIP with SA as dummy template has strong adsorption properties for GA, which has a good prospect of popularization and application in the separation and purification of GA.