Simultaneous determination of nine constituents in Jiuwei Gantai Capsules by HPLC-DAD / 中草药

ABSTRACT

Objective To establish HPLC-DAD method for the simultaneous determination of allantoin, ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, baicalin, schisandrin, curcumin, emodin and chrysophanol in Jiuwei Gantai Capsules (JGC). Methods The chromatographic separation was achieved on an Ultimate AQ-C18 column (150 mm × 4.6 mm, 5.0 μm) with mobile phase consisted of (0.1% phosphate)-(acetonitrile-methanol 20 80) for gradient elution, at the flow rate of 1.0 mL/min; The column temperature was 35 ℃. Results The linear ranges of allantoin, ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, baicalin, schisandrin, curcumin, emodin, and chrysophanol were 1.6—160.0 μg/mL (r = 0.999 7), 1.2—120.0 μg/mL (r = 0.999 6), 1.2—120.0 μg/mL (r = 0.999 6), 0.4—40.0 μg/mL (r = 0.999 2), 4.0—400.0 μg/mL (r = 0.999 8), 0.4—40.0 μg/mL (r = 0.999 1), 0.16—16.0 μg/mL (r = 0.999 1), 0.08—8.00 μg/mL (r = 0.999 0), and 0.2—20.0 μg/mL (r = 0.999 2), respectively. The average recoveries (n = 6) were 99.3% (RSD = 0.6%), 100.2% (RSD = 0.5%), 99.8% (RSD = 0.7%), 98.3% (RSD = 1.1%), 99.9% (RSD = 0.3%), 97.8% (RSD = 0.9%), 97.8% (RSD = 1.4%), 102.2% (RSD = 1.5%), and 101.9% (RSD = 1.2%), respectively. The content of the allantoin, ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, baicalin, schisandrin, curcumin, emodin, and chrysophanol in nine batches were 3.634—3.655, 2.523—2.611, 2.405—2.424, 0.802—0.829, 10.362—10.623, 0.901—0.921, 0.334—0.366, 0.142—0.160, and 0.462—0.479 mg/g, respectively. Conclusion The method is accurate, sensitive, credible, and repeatable, which can be applied to the quality control of JGC.