Applicability of membrane separation technique for concentrating anti-inflammatory compounds of Cornus officinalis Decoction based on anti-rheumatoid arthritis activity / 中草药

ABSTRACT

Objective To investigate the anti-inflammatory effect of Cornus officinalis (CO) Decotion and its refined solutions from membrane separation by using 0.05 μm inorganic ceramic membrane (CO-0.05) and 10K polysulfone hollow fiber membrane (CO-10K), and evaluate the applicability of the membrane separation technique for concentrating the anti-inflammatory compounds of C. officinalis Decotion. Methods Inflammatory model of interleukin (IL)-1β and tumor necrosis factor (TNF)-α stimulated human fibroblast-like synoviocytes (HFLS) was prepared. The CCK-8 assay and ELISA were applied to detect the effects of C. officinalis Decotion and its refined solutions on the viability of HFLS and the secretion of inflammatory cytokines, respectively. Moreover, the animal model of adjuvant arthritis (AA) was used. The SD rats were divided into six groups control group, model (AA) group and AA groups intragastrically receiving CO (120 mg/kg), CO-0.05 (120 mg/kg), CO-10K (120 mg/kg) and TGP (0.125 mg/kg) with daily treatments for 23 days. The weight and paw swelling of rats in different groups were detected. The ELISA was used to detect secretion levels of prostaglandin E2 (PGE2), IL-1, IL-6, and TNF-α in serum. Results The production of vascular endothelial growth factor (VEGF) induced by IL-1β/TNF-α were significantly inhibited with C. officinalis Decotion and its refined solutions by membrane separation treatment (P < 0.05, 0.01, 0.001). C. officinalis Decoction and the refined solutions significantly ameliorated paw swelling and increased weight gain of AA rats (P < 0.05, 0.01, 0.001), and reduced the secretion of TNF-α, PGE2, IL-1, IL-6 in serum (P < 0.001). By comparing the inhibition efficiency of inflammatory cytokines by inorganic ceramic membrane refined solution and polysulfone hollow fiber membrane refined solution, the polysulfone hollow fiber membrane refined solution exhibited better anti-inflammatory activity. Conclusion Both of refined solutions of C. officinalis Decotion from inorganic ceramic membrane and polysulfone hollow fiber membrane separation exhibited dramatically anti-inflammtory activity. Moreover, the polysulfone hollow fiber membrane was more applicable for concentrating the anti-inflammatory compounds of C. officinalis Decotion.