Mechanism of herb pair containing Scutellariae Radix and Coptidis Rhizoma on treatment of type 2 diabetes mellitus based on network pharmacology / 中草药

ABSTRACT

Objective: To investigate the mechanism of type 2 diabetes mellitus (T2DM) treatment by drug pair containing Scutellariae Radix and Coptidis Rhizoma. Methods: The chemical composition and targets of Scutellariae Radix and Coptidis Rhizoma were searched by the analysis of traditional Chinese medicine system pharmacology platform (TCMSP), and T2DM related genes were searched by OMIM, TDD, Drugbank, and Digsee databases. UniProt database was used to query the target-related genes. Cytoscape 3.2.1 was used to construct compound-targets (genes) networks and protein-protein interaction (PPI) network to screen out the key targets. Finally, the mechanism of its action was studied by DAVID analyzing enrichment of gene ontology (GO) function and pathway enrichment analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG). Results: The compound-target network contained 42 compounds and 213 corresponding targets, and the key targets involved PTGS2, PTGS1, HSP90AA1, HSP90AB1, NOS2, etc. The PPI core network contained 15 protein, and key protein involved in TNF, IL-6, INSR, etc. The functional enrichment analysis of GO obtained 108 GO items (P < 0.05), of which there were 87 biological processes (BP) items, 9 molecular function (CC) items, and 12 related items of cell composition (MF). There were 24 signal pathways (P < 0.05) in the KEGG pathway enrichment screening, involving insulin resistance, T2DM and insulin signaling pathway and so on. Conclusion: The active components of Scutellariae Radix and Coptidis Rhizoma regulate inflammatory cytokines and act on insulin receptor in the treatment of T2DM mainly through DPP4, PPARG, IL6, PPARD, TNF, and other targets.