Rule of endophytic fungi on changes in content of plant hormones and enzyme during seed germination / 中草药

ABSTRACT

Objective: To study the Methods: of endophytic fungi to break the dormancy of Acanthopanax senticosus seeds and the changes of endogenous hormones and enzymes. Methods: The seeds of A. senticosus were stimulated with endophytic fungi, then treated under thermophilic stratification. The content of endogenous hormones such as GA3, ABA, IAA, IBA, and SA, in the seeds of A. senticosus were tested by HPLC. And the changes of its in vivo enzymes (CAT, SOD, POD, and MDA) activity were tested. Results: Five strains of endophytic fungi from A. senticosus apparently promoted the germination of seeds. In the process of fluctuating temperature stratification, the content of GA3, IAA, IBA, and SA were increased, at the same time, the content of ABA was reduced. The activity of POD and MDA enzymes was significantly reduced, and the enzyme activities of CAT and SOD increased obviously. Conclusion: It is suggested that endophytes have significant effect on the content of seed hormones and enzymes, in addition endophytes could promote the initiation of seed germination.