Establishment of UPLC fingerprints of Zhidong Granules and determination of three constituents / 中草药

ABSTRACT

Objective To establish the UPLC fingerprints of Zhidong Granules (ZG) and determine the content of three constituents. Methods With baicalin as a reference peak, the UPLC fingerprints of 11 batches of samples were established. The analysis of ZG was performed on a 40 ℃ thermostatic Acquity HSS T3 column (150 mm × 2.1 mm, 1.8 μm), with the mobile phase comprising of acetonitrile-0.1% formic acid flowing at 0.3 mL/min in a gradient elution manner, and the detection wavelength was set at 254 nm. Results The UPLC fingerprint was established and 16 components were identified (gallic acid, asperuloside, methyl deacetylcyrrhizin, chlorogenic acid, genipin 1-gentiobioside, geniposide, paeoniflorin, chrysin-6-C-β-D-glucopyranosyl-8-C-α-L- arabinopyranoside, chrysin-6-C-α-L-arabopylanose-8-C-β-D-glucoside or isomer, baicalin, baicalin isomer, oroxylin A-7-O- glucuronide, wogonoside, wogonin, and schizandrin). The similarity of 11 batches of preparations were at least 0.98. Baicalin, paeoniflorin, and geniposide showed good linear relationships within the ranges of 12.15-388.80 μg/mL, 6.52-209.00 μg/mL, and 8.38-268.00 μg/mL, whose average recoveries (RSD) were 100.98% (3.04%), 100.49% (0.60%), and 100.55% (2.73%), respectively. The content of baicalin in the 11 batches of preparations was between 7.46 and 12.40 mg/g, the content of geniposide was between 7.01 and 13.27 mg/g, and the content of paeoniflorin was between 7.68 and 12.76 mg/g. Conclusion The method is accurate, simple, stable, and reliable, and can be used for quality control of ZG.