Lipidomics study of protective effect of Salvia miltiorrhiza and Ligusticum chuanxiong on rats with focal cerebral ischemia injury based on UPLC-Q/TOF-MS / 中草药

ABSTRACT

Objective To study the regulating effect of freeze-dried substances of Salvia miltiorrhiza and Ligusticum chuanxiong Decoction on lipid metabolism abnormality in focal cerebral ischemia rats. Methods The focal cerebral ischemia rat model was established by monofilament method. The rats were randomly divided into normal group, sham operation group, model group, and drug treatment group. Plasma was collected after the last dosage and UPLC-Q/TOF-MS was used to study the plasma lipidomics. The lipidomics data were analyzed by orthogonal partial least square discriminant analysis (OPLS-DA), and the lipid metabolites changes were examined before and after the intervention of S. miltiorrhiza and L. chuanxiong. Results The focal cerebral ischemia rat model was successfully repeated. The S. miltiorrhiza and L. chuanxiong Decoction freeze-dried substances obviously reversed the abnormal lipid metabolism profile in the focal cerebral ischemia rat model. The plasma lipid biomarkers of ischemia injury rat were PS (210/00), PG (120/170), C16 sphinganine, phytosphingosine, PE [181 (9Z)/00], PC (161/20), PC (00/180), PC (161/00), PC (160/00), PC (182/00), PC (181/00), PC (180/00), and PC (205/00), respectively. Conclusion S. miltiorrhiza and L. chuanxiong Decoction freeze-dried substances have protective effects on cerebral ischemia injury, which may be related to the regulation of abnormal lipid metabolism, especially for phosphatidylcholines (PCs).