Rapid discovery of acetylcholinesterase inhibitors from natural organism / 中草药
Chinese Traditional and Herbal Drugs
; (24): 5756-5760, 2018.
Article
in Zh
| WPRIM
| ID: wpr-851469
Responsible library:
WPRO
ABSTRACT
Objective To establish an effective method to rapidly detect acetylcholinesterase inhibitors from natural products, and provide support for the treatment of Alzheimer’s disease with Chinese medicine and its active ingredients. Methods The activity of restraining acetylcholinesterase of crude extracts of a variety of medicinal plants were evaluated by applying Ellman’s colorimetric, and the better activity of the extract sample solution was filtered out. The active ingredients of medicinal plants were identified and separated by the application of AS/UF-UPLC-MS. Results According to the experimental data, Corydais yanhusuo, Phellodendron chinense, Uncaria rhynchophylla, and Gynura segetum had significant restraining acetylcholinesterase activity. Rapid screening showed that 3α-dihydrocadambine, 3α-isodihydrocadambine, rhyncholphylline, isorhynchophylline, corynoxine, and isocorynoxine were active ingredients in U. rhynchophylla. Conclusion The results suggest that it is feasible to screen acetylcholinesterase inhibitors from natural organism plants. Using AS/UF-UPLC-MS methods, leading compounds for the development of new drugs for Alzheimer’s disease would be developed and provided.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Traditional and Herbal Drugs
Year:
2018
Type:
Article