Effectsofcerebrospinalfluidcontainingdrugsfortonifyingspleenandstomachonactivityandmigrationofrathippocampalneuralstemcells / 中草药

ABSTRACT

To study the effects of prescriptions including Ginseng, DabuyuanDecoction, and XixinDecoctionfor tonifying spleen and stomach on the activity and migration ability of neural stem/progenitor cells (NSPCs) induced by β-amyloid protein (β-amyloid),and investigate the effect of hippocampal neuronal repair and regeneration. Methods NSPCs were isolated from fetal rats of 14-16 d gestation and identified. It was randomly divided into seven groups blank group, blank group treated with Aβ1-42, control group of normal cerebrospinal fluid, control group of Aricept, prescription of Tonifying Spleen and Stomach and Yuanqi (Ginseng, Dabu Yuan Decoction, and XixinDecoction) Protection groups. The third generation of neural stem cells were treated with 25 μmol/L Aβ1-42 for 48h. The activity of NSPCs in each group was detected bytranswell assay and CCK-8 assay and themigration was detected by scratch test. The content of reactive oxygen species (Ros) in the cells was detected by flow cytometry. ResultsBy immunofluorescence staining, NSPCs specific protein Sox2 staining was positive. The CCK-8 detection results showed that the activity of NSPCs in hippocampus was significantly increased in the groups of Tonifying Spleen and Stomach YuanqiPrescription (P< 0.01); The results of Transwell experiment and scratch test showed that the migration ability of NSPCs was significantly increased (P< 0.05, 0.01). The flow cytometryshowed that the content of ROS in the NSPCs of spleen and stomach prescription groups decreased significantly (P< 0.01). Conclusion The prescriptionsfor invigorating spleen and stomach promoted the activity and migration of NSPCs in rat hippocampal neural stem cells. The oxidative stress induced by Aβcan be antagonized and its active ingredients can easily penetrate the blood-brain barrier to exerta stronger neuroprotective effect.