Effect of decoction and drug-containing serum of sweated and crude Radix Dipsaci on proliferation of MG-63 cells and osteoblasts / 中草药

ABSTRACT

Objective To investigate the effect of decoction and drug serum of sweated and crude Dipsaci Radix on the proliferation of human osteoblast-like cells (MG-63) and osteoblasts. Methods MG-63 cells and osteoblasts were co-cultured with decoction and rat serum containing Dipsaci Radix before and after “sweating”. The cell proliferation was detected by MTT method and the ALP activity of osteoblasts was detected by nitrophenyl phosphate method. Results Both decoction and drug-containing serum can significantly promote the proliferation of MG-63 cells and osteoblasts (P < 0.01), and significantly increase the ALP activity of osteoblasts (P < 0.01). Moreover, sweated group were generally better than or non-inferior to crude group at the same dose concentration of two administration methods. Conclusion Combined with component studies, it can be inferred that the changes in composition before and after "sweating" affect its role in promoting cell proliferation and differentiation, with view to laying the foundation for further research.