Metabolomics analysis and quantitative determination of five components in Malus leaves / 中草药

ABSTRACT

Objective The aim of this experiment is to compare the chemical composition of Malus leaves from different origins, which can be as a fundamental research for the comprehensive utilization and resource development. Methods A UPLC-DAD method has been developed for the determination of five compounds (phlorizin, trilobatin, phloretin, rutin, and quercitrin) in Malus leaves, and UPLC-QTOF-MS based metabonomics was applied to the comparison among different groups from different origins and species. Results There were significant difference in the content of five compounds, of which phlorizin was widely contained and its content was relatively high in the range of 1.01% -17.15%. Phloretin, rutin, and quercutrin were also widespread, while the trilobatin was only in Ser. Baccatae (Rehd.) Rehd and Sect. Sorbomalus Zabel, which was not detected in the cultivating-varieties. The results of metobonomics showed that the chemical composition in leaves of Malus between Ser. Pumilae (Rehd). Rehd and Ser. baccatae (Rehd). Rehd were clearly distinguished, and 26 discrimination markers as the basis for distinction were identified; The chemical composition of Malus leaves from different sources of origins were different while the wild species were similar to the cultivating- varieties. Conclusion The Malus leaves generally contain phlorizin and other polyphenols, which are the potential drugs and health products resources; There is certain difference in chemical composition and content of Malus leaves, which provide the scientific basis for the next utilization; Malus leaves are so rich and renewable that are worthy of further research and development.