HPLC and physical fingerprint of Chuanxiong Rhizoma standard decoction / 中草药

ABSTRACT

Objective To prepare 15 batches of Chuanxiong Rhizoma standard decoction, and establish HPLC fingerprint and physical fingerprint for quality evaluation. Methods Fifteen batches of Chuanxiong Rhizoma standard decoction were prepared according to preparation conditions of the traditional Chinese medicine (TCM) standard decoction in “Technical Requirements for Quality Control and Standard Formulation of Prescription Granules of TCM (draft for soliciting opinions)”. The mobile phase of HPLC consisted of 0.6% aqueous solution of phosphoric acid and acetonitrile with gradient elution, column temperature of that was 30 ℃, flow rate of that was 1.0 mL/min and detection wavelength of that was 324 nm. Physical fingerprint that consisted of nine secondary indexes (relative homogeneity index, intergranular porosity, Karl index, loose density, vibrating density, dry weight loss, hygroscopicity, Hausner ratio, and angle of repose) was established. Results There were 13 common peaks in the HPLC control fingerprint, and three of them were identified as chlorogenic acid, caffeic acid, and ferulic acid respectively. Taking ferulic acid as a reference peak, the relative retention time and relative peak area of each co-owned peak were analyzed, and the limits of relative retention time and relative peak area of each co-owned peak were specified. The HPLC and physical fingerprint were established, and the similarity should greater than 0.9. Conclusion The HPLC and physical fingerprint of the Chuanxiong Rhizoma standard decoction can be used as reasonable and stable index to evaluate the quality of Chuanxiong Rhizoma standard decoction. Which provides a reference for the preparation and quality control of Chuanxiong Rhizoma dispensing granule.