Optimization of fresh-cuting process and its pharmacodynamics of Aurantii Fructus / 中草药

ABSTRACT

Objective To study the effect of different cutting time and drying methods on the index components of pharmacopeia in different fresh-cutting Aurantii Fructus, and investigate the difference of component and efficacy between the preferred fresh-cutting Aurantii Fructus and the traditional slices. Methods The HPLC system consisted of a Diamonsil C18 (250 mm × 4.6 mm, 5 μm) with acetonitrile/phosphoric acid water (20∶80, pH 3.0) as the mobile phase, the flow rate was 1.0 mL/min, column temperature was 30 ℃, detection wavelength was 283 nm, and the injection volume was 20 μL. The content of naringin and neohesperidin in fresh-cutting Aurantii Fructus with different processing methods was determined to optimize the cutting time and drying method. At the same time, the effects of sclices from fresh-cutting and traditional process on the small intestine propulsion rate, gastric emptying rate, gastrin, and hemorheology were determined. Results The content of naringin and neohesperidin of the fresh-cutting Aurantii Fructus that dried in sun was higher than that dried at 60 ℃ in oven at the corresponding days from the same origin, and the highest content was from fresh-cutting slices dried in sun for 3 d. There was no significant difference in the indexes of small intestine propulsion rate, gastric emptying rate, and gastrin between the preferred fresh-cuting Aurantii Fructus and the traditional ones, but the indexes of hemorheology showed significant difference (P < 0.05). Conclusion The preferred fresh-cutting Aurantii Fructus (processed by half cutting horizontally, drying for 3 d, and then cutting into thin slices and drying in the sun) showes no significant difference in gastrointestinal motility indexes when compared with the traditional ones.