Effect of Longzuan Granules on cardiac function and inflammatory reaction mediated by NF-κB in myocardial infarction rats / 中草药

ABSTRACT

Objective To observe the effects of Longzuan Granules (LZG) on cardiac function and the inflammatory reaction mediated by nuclear factor-κB (NF-κB) in myocardial infarction (MI) rats, and explore the protective effect on MI and its mechanisms. Methods The MI model was established by ligating the left anterior descending coronary artery of SD rats, sham group was only threading without ligation. The rat models were randomly divided into five groups model group, Valsartan group (10 mg/kg), high-, medium-, and low-dose LZG (19.00 g/kg, 9.50 g/kg, and 4.75 g/kg) groups, with 10 rats in each group, ig administered once daily for 28 consecutive days. Changes in left ventricular function (EF%, FS%, and ± dp/dtmax) were detected by echocardiography and hemodynamics. The levels of inflammatory cytokines IL-6, IL-1β, TNF-α, and CRP in serum were measured by ELISA, and the mRNA expressions of IL-6 and TNF-α in cardiac tissues were detected by qRT-PCR. The protein expression levels of NF-κB P65 and p-P65 were detected by Western blotting. Results Compared with the model group, LZG significantly improved left ventricular function in rats; The serum levels of IL-6, IL-1β, TNF-α, and CRP decreased significantly (P < 0.05, 0.01) in LZG groups, the mRNA expressions of IL-6 and TNF-α in the cardiac tissues was also decreased (P < 0.05, 0.01). LZG significantly decreased the protein expression levels of NF-κB p-P65 (P < 0.05, 0.01). Conclusion LZG can improve myocardial injury, enhance the left ventricular function, and reduce the inflammatory reaction in MI rats, and its mechanism may be related to the inflammatory response through NF-κB signaling pathway.