Analysis on chemical constituents from rat kidney tissue of Juglans mandshurica based on UPLC-Q-TOF/MS / 中草药

ABSTRACT

Objective To analyze the chemical constituents from rat kidney tissue of Juglans mandshurica by UPLC-Q-TOF/MS. Methods The kidney tissue was collected after oral administration of ethanol extract of J. mandshurica. The gradient elution was performed using a Waters Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), 0.1% formic acid water (A), and 0.1% formic acid acetonitrile (B) mobile phase system. An electrospray (ESI) ion source was used for mass spectrometry to collect data in positive ion mode. Combined with Peakview 2.0/masterview 1.0 and Metabolitepilot data analysis software, the kidney tissue components of J. mandshurica were identified by comparing the retention time, isotope kurtosis ratio, exact mass of the parent ion, and MS/MS fragment. Results Twenty-four chemical constituents including 16 prototypic components and eight metabolites were identified from rat kidney tissue, which contains 12 naphthoquinones, five flavonoids, three diarylheptanoids, and four triterpenoids. Conclusion The prototype components and metabolites of kidney tissue of J. mandshurica were determined. In further study, it provides reference for the safety and effectiveness of the treatment of J. mandshurica and provides a methodological reference for its in vivo composition and tissue distribution.