Identification of Rhodiola quadrifida and Rhodiola crenulata based on NMR fingerprint and chemical pattern recognition method / 中草药

ABSTRACT

Objective A method for classification and identification of Rhodiola quadrifida and Rhodiola crenulata was established based on nuclear magnetic resonance 1H-NMR fingerprints-chemical pattern recognition technique. Methods Using high resolution (600 MHz) NMR fingerprints pattern technique, the total component information 1H-NMR fingerprint of R. quadrifida and R. crenulata was determinated, combined with the similarity analysis, hierarchical cluster analysis, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) methods for chemical pattern recognition analysis. Results 1H-NMR fingerprints techniques combined with chemical pattern recognition analysis was an effective method to discriminate and identify R. quadrifida and R. crenulata. The difference of the 1H-NMR fingerprint of R. quadrifida and R. crenulata was obvious, which truly and comprehensively reflected the characteristic components and internal qualities of Rhodiola. The main different components of R. quadrifida and R. crenulata were terpenoids and flavonoids, in particular, crenulatin of the terpenoid was a characteristic ingredient in the identification of R. quadrifida and R. crenulata, which can be used as the identification and classification index of R. quadrifida and R. crenulata. Conclusion 1H-NMR fingerprints techniques combined with chemical pattern recognition analysis method is an effective method for classification and identification of Rhodiola, which lays the foundation for variety identification and quality evaluation of medicinal plants of Rhodiola.