Isolation and purification of polysaccharides from leaves of Eriobotrya japonica and its monosaccharide composition / 中草药

ABSTRACT

Objective: To determine the relative molecular weight (Mw), monosaccharide composition, and structures of polysaccharides from the leaves of Eriobotrya japonica. Methods: The polysaccharides (PEL60) were extracted from the leaves of E. japonica with hot water, followed by precipitating with ethanol, freezing, and drying. PEL60-A was purified by DEAE-Sepharose Fast Flow ion column and Sephacryl S 500 High-Resolution gel column chromatography from PEL60 of the leaves of E. japonica. The purity and molecular weight of PEL60-A was detected by high performance liquid chromatography (HPLC), and the monosaccharide composition and molecular ratio of the PEL60-A was analyzed by gas chromatography-mass spectrometry (GC-MS). Results: The PEL60-A, with Mw of 2.69 × 105. The composition of monosaccharide was mainly L-rhamnose and L-fucose and a small amount of D-xylose, D-mannose, D-glucose, and D-galactose with the molar ratio of 0.781.000.150.090.110.19. Conclusion: The study on isolation, purification, and preliminary identification of monosaccharide composition from leaves of E. japonica provided the important scientific theoretical basis for the fine processing of the leaves of E. japonica.