Effects of Banxia Xiexin Decoction on intestinal flora and inflammatory factors of diabetic gastroparesis rats / 中草药

ABSTRACT

Objective: To observe the effects of the Banxia Xiexin Decoction (BXD) on the intestinal flora and inflammatory factors of diabetic gastroparesis (DGP) rats. Methods: The high fat and sugar feeding combined with intraperitoneal injection of STZ were used for the preparation of DGP rats model, which were randomly divided into model group, the metformin (1.80 g/kg) group, the low (2.55 g/kg), medium (5.10 g/kg), and high (10.20 g/kg) dose group of BXD, and the blank group with 10 rats in each group. Four weeks of continuous ig administration of fasting blood glucose (FBG), gastric residual rate, intestinal propulsion rate, serum D-xylose level, intestinal flora, serum endotoxin, immunoglobulin A, and inflammatory factors were detected. Results: Compared with the blank group, the body weight, intestinal propulsion rate, flora (bacteroides, bifidobacterium, enterococcus, and lactobacilli) amount, IgA, and IL-10 in DGP model were significantly reduced (P < 0.01); FBG, gastric residual rate, serum D-xylose, enterobacter number, endotoxin, and TNF-α were significantly increased (P < 0.01); Compared with model group, high dose BD group of body weight, intestinal propulsion rate, flora (bacteroides, bifidobacterium, enterococcus, and lactobacilli) amount, IgA, and IL-10 level in the low, medium, and high dose group of Banxia Xiexin decoction were increased significantly (P < 0.05, 0.01), FBG, gastric residual rate, serum D-xylose, enterobacter number, endotoxin and TNF-α were significantly decreased (P < 0.05, 0.01). The FBG, intestinal propulsion rate, bacteroides number, and IgA level in the low-dose group were less affected. Conclusion: BXD can effectively adjust the various indexes of DGP model rats, including improving the intestinal flora proportion, reducing endotoxin, inhibiting inflammation factors, and improving the intestinal barrier function, and the potential mechanism may be related to regulation of intestinal bacteria and inflammation factors.