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Preparation and in vitro evaluation of folic acid-albumin nanoparticles co-loaded with paclitaxel and nanosilver / 中草药
Chinese Traditional and Herbal Drugs ; (24): 2786-2792, 2018.
Article in Chinese | WPRIM | ID: wpr-851895
ABSTRACT
Objective Paclitaxel and nanosilver were co-encapsulated into folic acid-albumin nanoparticles to increase the toxicity and uptake of the drug and improve the targeting ability of tumor that highly expressed folic acid (Fa) receptor. Methods The mean Fa binding number of bovine serum albumin (BSA) was determined by ultraviolet absorption method. The nanoparticles were prepared through self-assemble method and then its shape, partical size, Zeta potential, and encapsulation efficiency were characterized with the dynamic light scattering (DLS) and transmission electron microscope (TEM). In addition, the cellular uptake of nanoparticles and the in vitro anti-tumor effect of drug-loaded nanoparticles were investigated on the KB cells model of oral epithelial carcinoma. Results Ultraviolet absorption results showed that the average Fa binding number of Fa-BSA was 11. The nanoparticles were discrete and uniform spheres with the average size of (98.20 ± 3.58) nm and Zeta potential of (-39.90 ± 1.98) mV. Cellular uptake experiments showed that folate-modified albumin nanoparticles were more easily taken up by KB cells. Cytotoxicity and apoptosis assay indicated that the modification of folic acid and co-loaded nanosilver could increase the inhibition of tumor cell proliferation and promote KB cells apoptosis. Conclusion The prepared nanoparticles have small particle size and high encapsulation efficiency, which can enhance the ability of the nanoparticles to be uptaken and the toxicity of the drug-loaded nanoparticles against tumor cells.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2018 Type: Article