In vivo pharmacokinetic study on naringin and paeoniflorin of Painong Powder with or without Platycodonis Radix in rats / 中草药

ABSTRACT

Objective To compare the pharmacokinetic effect of Platycodonis Radix in Painong Powder on naringin and paeoniflorin in rats, and study the compatibility effects of Platycodonis Radix. Methods A total of 12 healthy male SD rats were randomly divided into the Painong Powders full formula group and Painong Powder without Platycodonis Radix group; The solution of Painong Powders full formula and Painong Powder without Platycodi Radix was ig administrated to rats at dose of 16 g/kg. The blood was collected from theretinal vein plexus of rats at predetermined time and pre-prepared with liquiritin as internal standard (IS). UPLC-MS/MS was adopted to determine the contents of naringin and paeoniflorin. The chromatography was eluted with mobile phase consisted of 0.1% formic acid and methyl alcohol at a flow rate of 0.2 μL/min. ESI sources were adopted to scan in a negative ion scanning mode. Results After ig administration Painong Powder and Painong Powder without Platycodonis Radix, the main pharmacokinetic parameters of naringin were as follow: AUC0-t = (2 643.22 ± 277.75), (1 934.01 ± 371.98) μg∙h/L; Cmax = (597.57 ± 22.46), (477.13 ± 25.67) μg/L; CL = (821.96 ± 175.64), (542.21 ± 100.97) L/(h∙kg); Paeoniflorin showed AUC 0-t = (3 178.25 ± 235.97), (1 384.11 ± 320.11) μg∙h/L; Cmax = (816 ± 28.88), (402.67 ± 58.22) μg/L; CL = (1 102.27 ± 341.17), (482.59 ± 39.74) L/(h∙kg) (P<0.05). Conclusion Compared Painong Powders without Platycodonis Radix, the naringin and paeoniflorin in rat plasma showed similar pharmacokinetic changes after oral :201 administration Painong Powders full formula, such as the peak time was shortened, the rate of absorption, peak concentration, and the clearance were accelerated. This study proved scientific compatibility of Platycodonis Radix in Painong Powder and provided a reference for further study of the prescription compatibility regularity in Painong Powders.