Quality comparison study between wild and cultivated Astragali Radix based on absolute growth years / 中草药

ABSTRACT

Objective To compare the chemical composition and the pharmaceutical effect of anti-heart failure of wild commodity Astragali Radix (WAR) and cultivated Astragali Radix (CAR). Methods The contents of flavonoids and saponins in Astragali Radix samples were determined by HPLC-UV-ELSD. The intervention effects of wild commodity and cultivated Astragali Radix on traditional efficacy index and metabolomics were compared in rat model with heart failure induced by adriamycin. Results The contents of total flavonoids, saponin I, and saponin III in WAR were significantly higher than those in CAR, but the content of total saponins was the equivalent. Pharmacodynamics experimental results showed that both WAR and CAR could improve the general situation in rats, histopathology, cardiac function parameters, serum biochemical indicators, and BNP. However, compared with the model group, the intervention effects of the WAR on cardiac function parameters (EF, FS, and LVIDs), biochemical markers (CK) and serum BNP content were superior to the CAR, and showed significant differences. The principal component analysis showed that the metabolic profiles of the normal control group and the model group were clearly distinguished. A total of 14 potential biomarkers associated with heart failure were identified, which could be adjusted to varying degrees in WAR and CAR, of which 2-hydroxyisobutyric acid, acetone, pyruvate, and formate were significantly changed in the WAR group. Conclusion In this study, the intervention of WAR in heart failure rats was significantly better than that of CAR, which provided a scientific basis for the establishment of specification grading, the clinical rational drugs use, and the classification of Astragali Radix by pharmaceutical companies for Astragali Radix.