A new type of natural eutectic solvent for green extraction of total flavonoids from Polygonati Odorati Rhizoma / 中草药

ABSTRACT

Objective To investigate the total flavonoids extraction process, solvent extraction rate, solvent recovery, and utilization using a new green solvent that nature deep eutectic solvents (NADESs) extraction of total flavonoids from Polygonati Odorati Rhizoma. Methods The extraction technology of total flavonoids was optimized by response surface methodology. The extraction efficiency of NADESs was evaluated by literature comparison and NADESs was recovered by macroporous resin adsorption. Results The NADESs (mole ratio 15) synthesized by sodium acetate and lactic acid was the best medium for extraction. After optimization by response surface methodology, the extraction rate of total flavonoids reached 20.13 mg/g at the best extraction condition of water 22%, extraction time 51 ℃, extraction time 21 min and liquid solid ratio 21 mL/g. The extraction rate of NADESs was significantly higher than the existing methods reported in the literature. The recovery experiments showed that the adsorption efficiency of NAK-9 on total flavonoids (88.67%) was significantly higher than that of D-101 (77.33%), and AB-8 (79.15%). Using methanol as solvent, the total flavonoids in Polygonati Odorati Rhizoma desorption rate was 80.46%. The recovered NADESs can be used for the extraction of total flavonoids in the next round, the extraction rate was 18.79 mg/g, and the recovery and utilization rate of solvent is 94.56%. Conclusion NADESs has the advantages of high efficiency, green environmental protection and can be reused as extraction medium, can be used for the extraction of total flavonoids from Polygonati Odorati Rhizoma. Meanwhile, the result provides a new idea that NADESs as an extraction medium for the extraction of other effective components of traditional Chinese medicine.