Optimization of extraction process of Wenfei Jiangzhuo Recipe based on pharmacodynamic screening and orthogonal experiment / 中草药

ABSTRACT

Objective To screen the extraction process of Wenfei Jiangzhuo Recipe (WJR) by pharmacodynamics and acute toxicity test, and to optimize it by orthogonal experiment to determine the best extraction process of WJR. Methods Acute toxicity test was carried out on different WJR samples. The improvement of learning and memory of vascular dementia (VD) rats was used as the efficacy index, and the results of the acute toxicity and efficacy experiments determined the best process plan. Using contents and yield rate as index, orthogonal test was used to screen the amount of solvent, extraction time and extraction times. Results The results of acute toxicity test showed that all mice survived without obvious toxicity. The results of pharmacodynamic test showed that the best extraction plan of WJR was as follows Extracting white prepared lateral root of Aconite, Codonopsis Radix, and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle with water, and Notoginseng Radix et Rhizoma, Zingiberis Rhizoma, and wine processing Rhei Radix et Rhizoma with ethanol. The optimized extraction technology were as follows The former were 10-fold water reflux extraction for three times and 1 h each time, with white prepared lateral root of Aconite first reflux 2 h; The latter were 6-fold 70% ethanol reflux extraction three times and each time 1.5 h. Conclusion WJR can significantly improve the learning and memory ability of VD model rats. The optimized extraction conditions are reasonable and feasible, and can provide the basis for the subsequent development of WJR.