Screening of preponderant arbuscular mycorrhizal fungi species from Paris polyphylla var. yunnanensis seedlings based on biomass and active components / 中草药

ABSTRACT

Objective To screen preponderant arbuscular mycorrhizal (AM) fungi species from Paris polyphylla var. yunnanensis seedlings based on mycorrhiza viability, rhizome biomass, and active components. Methods After 28 exogenous species of AM fungi were inoculated in sterilized soil, the effects of the mycorrhiza viability of seedling, the biomass in rhizome, the total amount of polyphyllins in single whole seedling, the amount of polyphyllins in rhizome, and the amount of nucleosides in rhizome of P. polyphylla var. yunnanensis were observed by inoculation test in pot at room temperature. Results All the 28 AM groups formed well mycorrhizal structure, the mycorrhiza viability of P. polyphylla var. yunnanensis seedling was enhanced, but it showed preference. Most of the AM treatment groups could improve the biomass in rhizome, the contents and yields of polyphyllins increased, which implied that the accumulation of polyphyllins and nucleosides in P. polyphylla var. yunnanensis seedling were enhanced after inoculation. AM also changed polyphyllins and nucleosides allocation in different organs of P. polyphylla var. yunnanensis seedling in the secondary metablism, the total amount of four kinds of polyphyllins in rootlet were higher than those in rhizome, but the total amount of nine kinds of nucleosides in rhizome were higher than rootlet. Conclusion The performance of different AM strains in this study is of great reference for further improving the biological quantity and quality of the screening of preponderant AM fungi species from P. polyphylla var. yunnanensis seedlings. Gigaspora gigantean, Scutellospora calospora, Diversispora spurca, Dentiscutata heterogama, Septoglomus deserticola, and Ambispora leptoticha can use as the preponderant AM fungi species for the cultivation of mycorrhizal seedlings of P. polyphylla var. yunnanensis.