Identification of major chemical constituents in Ixeris chinensis and Sonchus brachyotus by UHPLC-Q Exactive Orbitrap-HRMS / 中草药

ABSTRACT

Objective: To identify the similarities and differences of the main chemical constituents between Ixeris chinensis and Sonchus brachyotus by UHPLC-Q exactive orbitrap-HRMS. Methods: The analysis was performed on an Agilent Poroshell 120 reverse phase column (100 mm × 3 mm, 2.7 μm). The mobile phase consisted of methanol and 0. 5% acetic acid, which was used for gradient elution, and the flow rate was 0.3 mL/min. Q exactive orbitrap-HRMS spectrometry was applied for the qualitative analysis under positive and negative ion modes, and ESI ion source was used for mass spectra. Results: The results indicated that nine compounds from the ethanol extract of I. chinensis and ten compounds from the ethanol extract of S. brachyotus had been identified by direct comparison in both positive and negative ion mass data, the element compositions analysis, and the data of the literature. Among them, there were nine compounds are the same, which were seven organic acids and two flavonoids compounds. Conclusion: The efficient separation ability of UHPLC and high sensitive detection of MS were used in this study, which will provide the evidences for evaluating the quality of I. chinensis and S. brachyotus and stabilizing the curative effect in clinic.