Correlation analysis on idiosyncratic hepatotoxicity of Zhuangguguanjie Wan and 27 cytokines based on related visualization / 中草药

ABSTRACT

Objective: To analyze the correlation of idiosyncratic hepatotoxicity of Zhuangguguanjie Wan (ZGW) with 27 cytokines. Methods: After 12 h fasting, SD rats were ig with ZGW at a single dose of 3.8 g/kg, and injected with lipopolysaccharide (LPS) at a dosage of 2.8 mg/kg via tail vein after 2 h. The rats were anesthetized with chloral hydrate after 10 h LPS administration, and then the blood samples were collected from the inferior vena cava and liver tissue was also obtained. Alanine aminotransferase (ALT) activity in serum and levels of 27 cytokines in liver tissue were tested. Correlation analysis of ALT and cytokines were performed using R 3.2.4 software. Results: Correlation analysis showed that there was a high positive correlation between ALT and macrophage inflammatory protein-1α (MIP-1α) or vascular endothelial growth factor (VEGF). There was a strong correlation among interleukin-18 (IL-18), interferon-inducible protein-10 (IP-10), interleukin-1α (IL-1α), and interleukin-1β (IL-1β). However, eotaxin regulated upon activation of normal T cell expression and secreted factor (RANTES), which has their own independence. Conclusion: Idiosyncratic liver injury induced by ZGW has a strong positive correlation with MIP-1α and VEGF, which provides a new experimental evidence for clinical medication safety and risk prevention of ZGW.