Study on accumulation rules of effective components in Ophiopogonis Radix of different growth phase based on HPLC specific chromatogram / 中草药

ABSTRACT

Objective: To explore the change features of Ophiopogonis Radix in the development process, and to find the accumulation rules of effective components in Ophiopogonis Radix. Methods: The method of chlorfluazuron formulation was analyzed by HPLC. DAD detector, column temperature was set at 30 ℃ and detection wavelength was 203 nm; Flow rate was set at 0.8 mL/min and sample size was 15 μL and the mobile phase was acetonitrile (A)-water (B) with gradient elution mode (0~20 min, 25% A →40% A; 20~50 min, 40%~50% A; 50~60 min, 50%~70% A; 60~70 min, 70% A; 70~80 min, 70%~100% A, 80~90 min, 100% A), at the volume flow rate of 1.0 mL/min. ELSD detector, column temperature was set at 35 ℃, drift tube temperature at 100 ℃ and the gas flow rate was set at 3.0 L/min. The sample size was 15 μL and the mobile phase was acetonitrile (A)-0.1% phosphoric acid water (B) with gradient elution mode 0~60 min, 35%~65% A, at the volume flow rate of 1.0 mL/min. Results: The main components in the chromatogram were both separated well under the two conditions. At 203 nm, there were 14 effective peaks, the main components were flavones whose representative was methylophiopogonanone A. There were 11 effective peaks in the condition of ELSD, and the main peak represent saponins whose representative was Ophiopogonin D. It could be judged that the accumulation law of the each component in Ophiopogonis Radix was inconsistent in the different growth phase according to the peaks area. The overall change tendency of the components is increase to peak value and keep balance and to a slight decrease after the balance. Conclusion: The accumulation rules of effective components in Ophiopogonis Radix could be reflected comprehensively by HPLC specific chromatogram.It could provide abundant information for the produce of Ophiopogonis Radix and quality control.