Simultaneous determination of six active components in Panax ginseng by QAMS method / 中草药

ABSTRACT

Objective: This study aimed to simultaneously determine six composition of Panax ginseng by quantitative analysis of multi-components with a single-marker (QAMS) in different paris. Methods: Phenomenex Luna C18 (250 mm × 4.6 mm, 5 μm) was used with mobile phase consisting of acetonitrile-0.1% phosphoric acid for gradient elution at a flow rate of 1.0 mL/min, The column temperature was 25 ℃ and the detection wavelength was 203 nm. Ginsenoside Rb1 was used as reference to establish its relative correction factor of Rg1, Re, Rc, Rb2, Rd, The contents of six components were determined by both external standard method and QAMS. and t test was used to evaluate the feasibility and applicability of QAMS. Results: In a certain linear range, the relative correction factor (RCF) was good, No significant differences were observed between the quantitative results of the two methods. Conclusion: It is feasible and suitable to evaluate the quality of Panax ginseng. It can provide a useful reference to quality control of multi-indexed components in Chinese herbs and traditional Chinese preparations.