Effects of Xiaoyan Decoction on TGF-β/Smad3/MMP-9 signaling pathway in SP cells / 中草药

ABSTRACT

Objective: To investigate the specific mechanism of Xiaoyan Decoction in the metastasis of non-small cell lung cancer. Methods: The detection method of Western blotting method and quantitative PCR Xiaoyan Decoction intervention expression of many inflammatory proteins and cytokines in the lower lung cancer stem Xiaoyan Decoction explore the possible mechanism of effect of non small cell lung cancer metastasis. Results: Western blotting detection showed that the expression of IL-6, TGF-β, p-Smad3, and MMP-9 protein in both experimental group and control group decreased significantly (P < 0.05), the difference was statistically significant; RT-PCR showed that the experimental group compared with the control group IL-6, TGF-β, TNF-α, MMP-2, alpha beta MMP-9 gene expression decreased significantly (P < 0.05), the difference was statistically significant. Conclusion: Xiaoyan Decoction mainly affects the metastasis of non-small cell lung cancer by inhibiting the transmission of TGF-β/Smad3 signaling pathway in the inflammatory microenvironment of lung cancer.