Simultaneous determination of nine components of Scutellaria Baicalensis in different habitats and growth years by HPLC / 中草药

ABSTRACT

Objective To establish an HPLC method for simultaneous determination of nine main chemical constituents in Scutellaria baicalensis (scutellarin, baicalin, scutellarin, wogonoside, apigenin, baicalein, wogonin, aspen, and melaleuca (A). Methods 0.5 g powders of S. baicalensis was extracted two times by ethanol (each for 30 min), and the extracts were merged into 50 mL. Separation was carried out on Ultrasil C18 chromatography column (250 mm × 4.6 μm, 5 μm), by using acetonitrile-0.1% formic acid water as the flow phase for gradient elution; with volume flow at 1 mL/min, detection wavelength at 274 nm and column temperature at 30 ℃. Results The linear relation of nine main active components measured in the range of mass concentration was good with perfect precision, repeatability and stability, the r value of which was all more than 0.999 5. The average recovery rate was 95.94%-99.88%, RSD was 0.68%-1.90%. Conclusion The HPLC method established for the simultaneous determination of nine main chemical compositions in S. baicalensis is accurate, simple, sensitive and specific, which can provide references for the quality evaluation of S. baicalensis.