Formulation optimization of asiaticoside loaded capsosomes by central composite design-response surface method / 中草药

ABSTRACT

Objective To fabricate the asiaticoside loaded capsosomes with CaCO3 as core (CASI) and establish the method for determination of entrapment efficiency, then the prescription of formulation and preparation process were screened with the entrapment efficiency as index. Methods An HPLC method was established to determine the contents of asiaticoside. CASI were prepared by co-precipitation method and layer-by-layer assembly technique. The encapsulation efficiency was determined by a proved centrifugation. In this study, the effect of concentrations of capsule material, pH and rotating speed on encapsulation efficiency was investigated. Results The encapsulation efficiency obtained by centrifugation was accurate and reliable. The optimized prescription was concentrations of 1 mg/mL capsule material with pH value 12, pH 7.9 of liposomes, two precursor layers, one liposome layer, rotating speed 500 r/min, and 15 min of reaction time. The CLSM images confirmed the structural integrity of the CASI. Conclusion This formulation endowed with high encapsulation efficiency, and the CASI observed by CLSM turned out to be globular shapes and was narrow in size distribution.