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Study on mechanism of differentiation of esophageal carcinoma Kyse30 cells induced by p-hydroxylcinnamaldehyde / 中草药
Chinese Traditional and Herbal Drugs ; (24): 610-618, 2018.
Article in Chinese | WPRIM | ID: wpr-852214
ABSTRACT
Objective To investigate the effects of p-hydroxylcinnamaldehyde (CMSP) on cell proliferation, migration, cell cycle and the expression level of malignant biomarkers, and to investigate the underlying mechanism of differentiation of esophageal carcinoma Kyse30 cells (ESCC cells). Methods The effect of different concentration of CMSP at 0, 10, 20, and 40 μg/mL on viabilities of ESCC cell lines (Kyse30, Eca109, and Kyse180) for 24, 48, and 72 h was determined by MTS assay. Optical microscope and scanning electronic microscopy (SEM) were used to observe the morphologic changes of Kyse30 cells. The effect of CMSP at different concentration on cell cycle distribution and apoptosis of Kyse30 cells was assessed by flow cytometry analysis. ELISA was used to detect the effect of CMSP on expression of tumor related antigens (CEA and SCC) and malignant biomarkers (IL-6 and MIC-1) in Kyse30 cells at protein secretion level. Influence of different concentration of CMSP on migration and invasiveness of Kyse30 cells were determined by colony-formation, wound healing and Transwell assays. Western blotting was used to evaluate the effect of CMSP on expression of protein biomarkers C-myc and N-myc of Kyse30 cells and the related proteins in RhoA-MAPK pathway. Results The proliferation of esophageal cancer cell lines (Kyse30, Eca109, and Kyse180) was significantly inhibited by CMSP in a dose- and time-dependent manner. The cell cycle Kyse30 was blocked in G0/G1 phase. After the treatment with CMSP, Kyse30 cells showed typical dendrite-like cellular protrusions, and the percentage of such elongated cells was significantly and progressively increased with the increase in CMSP concentration (P 0.05). CMSP could decrease the expression of CEA, SCC, IL-6, and MIC-1 both in protein secretion levels significantly in a dose- and time-dependent manner (P < 0.05, 0.01). Western blotting analysis showed that C-myc and N-myc proteins were all decreased significantly in Kyse30 cells after treatment with CMSP (P < 0.05). CMSP significantly inhibited the proliferation and migration ability of Kyse30 cells (P < 0.05) and induced cell differentiation; The protein levels of p-P38 was significantly increased (P < 0.01), while protein levels of ERK1/2, SAPK/JNK, and GTP-RhoA were obviously decreased in Kyse30 cells after treatment with CMSP (P < 0.01). Conclusion CMSP suppressed the proliferation and induced the differentiation of Kyse30 cells through regulating the RhoA-MAPK signal pathway, which might provide new potential strategies for ESCC treatment.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2018 Type: Article