Determination of six ingredients of gastrodin from gastrodia elata with quantitative analysis of multi-components by single marker / 中草药

ABSTRACT

Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for determining contents of six gastrodin compositions in Gastrodia elata, gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C and parishin E. Methods Separation was carried out on Agilent Zorbax SB-C18 Column (250 mm × 4.6 mm, 5 μm) by using acetonitrile (A)-0.05% Phosphoric acid aqueous solution (B) as the mobile phase, and a linear gradient elution was employed (0-5 min, 2% A, 5-20 min, 2%-30% A, 20-25 min, 30%-2% A) with detection wavelength at 220 nm, flow velocity at 1.0 mL/minand column temperature at 25 ℃. Five relative correction factors (RCFs) of the five compositions using gastrodin as the internal standard were applied to determine their contents in all samples It was established to determine the contents of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E by the external standard method (ESM) at the same time. The feasibility and accuracy of QAMS method were verified by comparing the content results determined by ESM and QAMS. Results Within the linear range, the RCFs of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E were 0.663, 1.278, 1.435 and 1.591, respectively, with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method. Conclusion The QAMS method is feasible and accurate for the determination of the six chemical compositions, and which can be used for quality control of G. elata.