Non-saponin constituents and their cytotoxicities from Trillium kamtschaticum / 中草药

ABSTRACT

Objective To investigate the chemical constituents and bioactivity of the moieties without hemostatic effects of Trillium kamtschaticum. Methods All compounds were isolated and purified by means of silica gel column chromatography, reverse phase C18 column chromatography, Sephadex LH-20, and recrystallization methods. Their structures were determined by physicochemical properties, and spectral data. The cytotoxic activity of selected compounds was also evaluated using the MTT method. Results Ten compounds were obtained from the moieties without hemostatic effects of T. kamtschaticum and their structures were identified as β-ecdysone (1), polypodine B (2), integristerone B (3), (9Z,15Z)-11,12,13-trihydroxyoctadeca-9,15-dienoic acid (4), (Z)-11, 12,13-trihydroxyoctadec-9-enoic acid (5), methyl (9Z,15Z)-11,12,13-trihydroxyoctadeca-9,15-dienoate (6), methyl (Z)-11,12,13- trihydroxyoctadec-9-enoate (7), 7,11-dimethyl-3-methylene-1,6-dodecadien-10β,11-diol-10-O-β-D-glucopyranoside (8), kaempferol- 3-O-α-L-arabinopyranosyl (1→6)-O-β-D-galacopyranoside (9), and 5-hydroxy-4-hydroxymethyl-2H-pyran-2-one (10), respectively. Compounds 1, 9, and 10 showed cytotoxic activities with IC50 values of (26.6 ± 1.3) μmol/L (DU145 cells), (16.2 ± 6.2) μmol/L (CEM cells), and (23.7 ± 1.2) μmol/L (HeLa cells), respectively. Conclusion Compounds 4—7 and 10 are obtained for the first time from Trillium genus, and compounds 1, 9, and 10 showed cytotoxic activities.