Correlation between chemical composition and pharmacological activity of Scutellaria baicalensis during its preparation process / 中草药

ABSTRACT

Objective Process design grants the quality connotation to products. To study the changes of main chemical ingredient of Scutellaria baicalensis preparation in the preparation process and the correlation with its pharmacological activity. To clarify the key step of S. baicalensis preparation process, and overall evaluate the short plate of the quality of preparation from the perspective of the design. Methods The preparation process (extracting-concentrating-drying-granulation) was simulated. HPLC analysis was employed to determine the contents of the chemical ingredients in each step of preparation process. Induction by LPS in mouse peritoneal macrophages of each intermediate was used as the model to determinate the anti-inflammatory activity. Cluster analysis and linear regression were used to analyze the correlation between its chromatogram and pharmacological activity. Results The content of chemical ingredients and anti-inflammatory activity of S. baicalensis were generally decreased on the whole, and the highest loss occurred in the concentrating-drying process. Conclusion Each step of the preparation process of S. baicalensis preparation has significant effect on the chemical ingredients and pharmacological activity. The concentrating and drying process may be the key process which could finally influence the quality of preparation.