Optimization of extraction and purification technology for phenolic acids and flavonoids in stems and leaves of Salvia miltiorrhiza / 中草药

ABSTRACT

Objective To study the optimum condition for the extraction and purification of phenolic acids and flavonoids in the stems and leaves of Salvia miltiorrhiza. Methods The contents of phenolic acids and flavonoids were determined by ultra performance liquid chromatography (UPLC). The extraction process was evaluated by single factor test and orthogonal design with yield of dry extract and the content of phenolic acids (including danshensu, protocatechuic acid, protocatechualdehyde, caffeic acid, salvianolic acid B, rosmarinic acid, and lithospermic acid) and flavonoids (including rutin, isoquercitrin, kaempferol-3-O-rutinoside, and astragalin) as index. The effects of extraction method, extraction solvent, ratio of material to liquid, extracting time, and extracting times on the extraction of stems and leaves of S. miltiorrhiza were investigated. Macroporous adsorption resin was used to purify the sample, and the purification process parameters were investigated to determine the optimum purification process. Results The optimum condition for the extraction of the stems and leaves of S. miltiorrhiza is that eight times of 50% ethanol for three times reflux extraction and 1 h for each time and AB-8 macroreticular resin was selected for the purification. Optimum process was as following:The concentration of sample solution was 1.0 g/mL; The loading quantity of the sample was 0.15 g dried extract of per gram; The resin column chromatography was eluted with 3 BV of 40% ethanol. Under these conditions, the total purity of phenolic acids and flavonoids could reach 40.83%. Conclusion The optimum technology is stable and feasible for the extraction and purification of phenolic acids and flavonoids in the stems and leaves of S. miltiorrhiza, and can provide reference for further development and utilization.