Spectrum-effect relationship of active fraction from Hedysarum polybotrys on osteoporosis / 中草药

ABSTRACT

Objective To study the correlation of anti-osteoporosis effect of Hedysarum polybotrys and HPLC fingerprint. Methods Osteoporosis model was established through ovary resection of female rats to select the best part after performing ig administration with different extracts of H. polybotrys. The fingerprints of ethanol extract of ten batches of H. polybotrys from different habitats were established by HPLC. The partial least squares regression (PLSR) analysis was used to explore the correlation between peak areas of HPLC fingerprints and efficacy. Moreover, the effects of active ingredient of H. polybotrys on the function of osteoblasts were observed. Results The ethanol extract of H. polybotrys was the most effective part. Nine common peaks of the active fraction were selected. Adenosine, calycosin and ononin in ethanol extract of H. polybotrys have been identified separately, among which adenosine and calycosin were positively correlated with the pharmacodynamic data, and ononin was negatively correlated with the effect of drug. Additionally, calycosin can increase osteoblast ALP activity. Conclusion The results indicate that anti-osteoporosis effect of H. polybotrys is related to various components, among which adenosine and calycosin have a certain contribution to this effect, and calycosin can also stimulate the differentiation of osteoblasts in vitro.