Cloning and bioinformatic analysis of chalcone isomerase gene in Lithocarpus polystachyus / 中草药

ABSTRACT

Objective: To understand the chalcone isomerase (CHI) gene expression by cloning it in Lithocarpus polystachyus. Methods: A full-length cDNA of CHI gene from Lithocarpus polystachyus (Lpr-CHI) was obtained by PCR cloning technique according transcriptomics sequences infromation, which bioinformatics analysis was carried out. The expression of CHI gene in different organs of Lithocarpus polystachyus was detected by qRT-PCR. Results: Lpr-CHI was 772 bp in full length with an open reading frame (ORF) of 696 bp, which encoded a protein with 231 amino acids. The protein did not contain a transmembrane domain and is localized in the cytoplasm. Lpr-CHI gene expression was found in different parts, and reached the highest in leaf, which was 9.75 times of the least gene expression in root. Conclusion: Lpr-CHI was obtained for the first time, and it was clear that the gene belongs to CHI type II. And the expression of Lpr-CHI in each organ was significantly different.