Effect of Epimedium brevicormon on Aβ25-35 induced SH-SY5Y cells injury / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 5206-5210, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-852323
ABSTRACT
Objective:
To compare the neuroprotective effect of different elution fractions and compounds of Epimedium brevicormon on SH-SY5Y cells injury induced by Aβ25-35 so as to screen pharmacologically fraction and compounds.Methods:
The isolation and purification of extract from E. brevicormon were performed on D101 macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative liquid chromatography. Hoechst 33342/PI double staining method was used to screen neuroprotective fractions and components.Results:
Compared to model group, all fractions and kaempferol-3-O-[α-L-rhamnopyranosyl (1→6)-β-D-galactoside]-7-O-α-L-rhamnopyranoside, icariin, epimedin A, and epimedin B from E. brevicormon could significantly increase the cells viability, except of 95% ethanol fraction.Conclusion:
E. brevicormon show some protective effect against Aβ25-35 induced SH-SY5Y cells injury. The CH2CI2-MeOH (51) extract from E. brevicormon has the most pharmacologically activity, followed by 30% ethanol and 50% ethanol extracts from E. brevicormon.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2017
Type:
Article
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