Changes of toxic substance in different phase of compatibility decoction Strychni Semen and Glycyrrhizae Radix et Rhizoma based on UPLC-MS technique / 中草药

ABSTRACT

Objective To study the mechanism of attenuated Strychni Semen (SS) and Glycyrrhizae Radix et Rhizoma (GRR) compatibility with the phase angle as the starting point. Methods The phase separation of SS single decoction and compatibility decoction was carried out by natural sedimentation, dialysis and high-speed centrifugation. UPLC-MS assay was used to study the differences in the distribution of brucine and strychnine in four different phases, and the change of the amount of toxic substances was analyzed. Results The compatibility of the decoction can be divided into four groups true solution group, colloidal solution group, suspension group, and sediment group. Brucine and strychnine were well separated under the selected chromatographic conditions. The linear relationship was good in the range of 0.020-2.008 and 0.022-2.236 μg (r = 0.999 8), and the recovery was 96.84% and 97.62%, which indicated that the method was good. The distribution of brucine and strychnine in the different phase of the single decoction showed the same variation regularity, colloid group > solution group > sediment group > suspended group; Compatibility decoction of different phase of brucine and strychnine also showed the same distribution, sediment group > colloidal group > suspension group > solution group. Conclusion The contents of toxic substances in SS were decreased after compatibility with GRR, especially for the strongest toxicity of strychnine, which mainly concentrated in the sediment components. Studies on the changes of toxic substances in phase states indicated that the sustained release effect of the sediments components after compatibility with GRR is the key factor of reducing toxicity and improving efficiency.